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Vitamin D, 25-OH Blood Test

Calcifediol Blood Test (Total Vitamin D, Vitamins D3 and D2 Levels)

CPT Code(s)



Vitamin D, 25-OH, Total; Vitamin D, 25-OH, D3; Vitamin D, 25-OH, D2


Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)

Reference Range(s)

Vitamin D, 25-OH, Total30-100 ng/mL
25-OHD3 indicates both endogenous production and supplementation. 25-OHD2 is an indicator of exogenous sources, such as diet or supplementation. Therapy is based on measurement of Total 25-OHD, with levels <20 ng/mL indicative of Vitamin D deficiency, while levels between 20 ng/mL and 30 ng/mL suggest insufficiency. Optimal levels are ≥30 ng/mL.

Vitamin D, 25-OH, D3Not established
Vitamin D, 25-OH, D2Not established

Clinical Significance

Measurement of serum 25-OH vitamin D concentrations provide a good index of circulating vitamin D activity in patients not suffering from renal disease. Lower than normal 25-OH vitamin D levels can result from a dietary deficiency, poor absorption of the vitamin or impaired metabolism of the sterol in the liver. A 25-OH vitamin D deficiency can lead to bone diseases such as rickets and osteomalacia. Above normal levels can lead hypercalcemia. This assay employs liquid chromatography tandem mass spectrometry to independently measure and report the two common forms of 25-hydroxy vitamin D: 25OH D3 - the endogenous form of the vitamin and 25OH D2 - the analog form used to treat 25OH Vitamin D3 deficiency.

Test FAQ

25-Hydroxyvitamin D [25(OH)D]

Alternative Name(s)

Vitamin D2, 25-hydroxy,25-Hydroxyergocalciferol (25OHD2),Vitamin D3, 25-hydroxy,D2 Vitamin,25-Hydroxyvitamin D3,25-OH-D3,Vitamin D, 25-Hydroxy,25-Hydroxycholecalciferol (25OHD3),25-OH-D2,D3 Vitamin,25-Hydroxyvitamin D2

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